Fluid thioglycolate media or thioglycolate broth is a multi-purpose enriched differentiating media used primarily to differentiate oxygen requirement levels of various organisms. Oxygen levels throughout the media are reduced via reaction with sodium thioglycolate. [2] This produces a range of oxygen levels in the media that decreases with increasing distance from the surface. This allows differentiation of aerobic, anaerobic, microaerophilic, and facultatively anaerobic organisms based upon growth at various levels in the media. As an example, anaerobic Clostridium will be seen growing only in lower portions of the media. O2 is often indicated in the media by a pink color change.
Thioglycolate broth is also used to recruit macrophages to the peritoneal cavity of mice when injected intraperitoneally.[3] It recruits numerous macrophages, but does not activate them.[3]
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